KMID : 0380420190430010006
|
|
Journal of Prventive Veterinary Medicine 2019 Volume.43 No. 1 p.6 ~ p.11
|
|
Effect of hemin and zinc on experimental colon carcinogenesis in mice
|
|
Choi Hyo-Suk
Kim Jun-Ho Nam Sang-Yoon Yun Young-Won Jeong Jae-Hwang Lee Beom-Jun
|
|
Abstract
|
|
|
Excessive intake of red meat has been associated with colon carcinogenesis. The effect of hemin and zinc on colon carcinogenesis was investigated in male ICR mice. After acclimation for 1 week, five-week-old mice received three s.c. injections (0-2nd weeks of the experiment) of azoxymethane (AOM; 10 mg/kg b.w.) weekly and were treated by 2% dextran sodium sulfate (DSS) in drinking water for the next 1 week to induce aberrant crypt foci (ACF). Mice were fed on AIN-76A purified rodent diet for 6 weeks. There were three experimental groups; control, hemin, and hemin + zinc groups. The daily oral doses of hemin and zinc were 534 mg/kg and 55 mg/kg b.w., respectively. After staining colonic mucosa with 0.2% methylene blue, the ACF, aberrant crypts (AC), and polyps were counted. Lipid peroxidation in liver was evaluated by thiobarbituric acid-reactive substances (TBARS) assay. The numbers of AC, ACF, large ACF (i.e., ¡Ã4 AC/ACF), and polyps in the hemin group were higher than those in the control group. In hemin + zinc group, the numbers of AC, ACF, large ACF were significantly lower compared to the hemin or control groups (p < 0.05), and the number of polyps was also significantly lower compared to that in the hemin group (p < 0.05). The TBARS level in the livers of the hemin + zinc group was significantly lower than that of the hemin group (p < 0.05). These results suggest that hemin enhances colon carcinogenesis and that co-treatment with zinc can protect against the induction of colon carcinogenesis.
|
|
KEYWORD
|
|
Zinc, hemin, colon carcinogenesis, aberrant crypt foci, lipid peroxidation
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|